Trim report

Seqomics Ltd. offers a number of ways to trim your sequence reads prior to assembly and mapping, including adapter trimming, quality trimming and length trimming. For each original read, the regions of the sequence to be removed for each type of trimming operation are determined independently according to choices made in the trim dialogs. The types of trim operations that can be performed are:

  1. Quality trimming based on quality scores
  2. Ambiguity trimming to trim off e.g. stretches of Ns
  3. Adapter trimming
  4. Base trim to remove a specified number of bases at either 3' or 5' end of the reads
  5. Length trimming to remove reads shorter or longer than a specified threshold

The trim operation that removes the largest region of the original read from either end is performed while other trim operations are ignored as they would just remove part of the same region.

The report includes the following:

 

Image trimreport
Figure 1. A report with statistics on the trim results.

 (Source: https://www.qiagenbioinformatics.com)